Ntawm no yog kuv kis tau li cas microlepidoptera, uas koj yuav ceev ceev daim ntawv ceeb toom no tsis yog ib txoj kev txheem. Kuv tau txais kev zoo nkaus li puzzled los yog puffs cua ntawm lwm micro-hais mav, tab sis, kuv lub nrws zoo hais lus rau lawv tus kheej. Txoj kev no yog muas thaum chiv thawj qhia rau kuv los Terry Harrison, tus creator ntawm Microleps.org. Rau qhov tshaj ntawd txheem txoj kev pom ntawm no (.pdf).
Firstly, Kuv hu me dua ~ 20 hli txhua yam ib “micro”, muaj tsis muaj taxonomic los yog phylogenetic tau cov kabmob no – ib tug npauj me me cia xwb. Kuv yuav mus rau txhua yam ntawm tus me me ntawm ib lub Nepticulidae rau ib tug Noctuidae me me quav txawm pin minuten pins. Rau feem coob txog leps ib qhov luaj li cas .20 yuav muaj txaus. Kuv zeem cia ridiculously koob fine .10 pins rau tus noj ntawm npaub me me quav; cov pins tsawg tshaj plaws yuav kuj tsis yooj yim mus nrhiav kev muag khoom. Me Noctuids (xws li ib co Acontiinae thiab Arctiinae – Cisthene thiab Crambidia) feem ntau tus pinned rau me me tag huv tibsi txheem pins no – ntawm #000-#1. Kuv yeej tuaj tsis nyiam siv cov pins me dua #1 saj zawg zog npaum li cas vim cov. Tuav ib tug npauj rau ib #000 koob tseem tsis zoo thiab muab ib daim ntawv lo yog ib nerve-racking feat. Ib ob chav mounted npauj nrog ib #3 tus PIN muaj qho yooj yim uas hloov thiab ntaus cim; tsis bent pins thiab tsis “flicks” uas yaj ua pa koj tshem me me. Tej ilv bodied npaub yuav tseem puav warrant ib tus pin loj txheem… tiam sis ob mounting ib lep yuav tsum yeej tseem yog tus koj nyiam kom peb (yam tsawg kawg hauv phau ntawv kuv).
Ntawm no yog kuv sau toolbox rau microleps.
Cov yas tas tseem ntawm BioQuip, Txawm li cas los koj tau kab mob ua npuas ncauj. Yuav tsum tau siv cov npuas dej siab-ceev koj yuav nrhiav tau. Kuv nqa ob peb me me los xij ntawm ib gel-ua npuas ncauj txawv tebchaw thiab tsis tau noj lub sij hawm yuav nrhiav tau cov ntawm no hauv Teb Chaws Asmeskas. Nws yog ob zaug kom tuab li plastazote thiab muaj ib qhov chaw smoother. “Cross-linked polyethelene” kuj muaj ib tug zoo match. Nws txawm li cas los tuav qhov tus pin thiab yuav tsum tau hloov li ob peb xyoos.
Kuv txoj kev:
- Tus PIN koj npauj tshiab hauv lub tshav pob siv minutens yog li koj xav tau lwm yam lepidoptera. Thaum kuv qhov muag yuav tswj qhov no tsis muaj magnification rau hnub, nws yuav tsis ua tau ntev ua ntej kuv yuav tau ib yam dab tsi nyiam qhov no. Koj yuav pin tus npauj dorsally los yog ventrally (Tus npauj kus yog thiab koj ntxig sharpened nawj ntsis ntawm tus ob forelegs). Qhov no ua rau tsis txawv nrog ib ob chav mount vim kuj unsharpened kawg yog zoo txaus rau pinning mus ua npuas ncauj.
- So kom txaus koj npaub siv kuv txoj kev nyob ntawm no. Kiag li tsis ethanol yuav tsum muab sau rau hauv cov chamber, tus nqi tsawg tshaj plaws yuav tsis so ib micro txig. Micros feem ntau yuav tau so thiab kis muaj me ntsis teeb meem, thiab nws yuav siv xwb 6-12 sij hawm no after. Txawm li cas los, npaub ilv thiab tsawg tej zaum yuav yeej tsis tsim nrws zoo yog tias lawv tsis tshiab. Yog hais tias koj yeej muaj txhua, sau qhov tsawg tshaj plaws npaub ciaj sia nyob rau hauv vials, thiab cia rau hnub tom qab kis. Vials iav uas preferable vim hais tias tus zoo li qub xwb uas yuav ua tau nyob hauv yas ua ib tug npauj lo thiab mos nplai. Dispatch ntawm tus npauj hauv koj lub freezer los yog nrog ib tug me quav poob ntawm acetate thiab npaj mus.
- Maj mam muab taub ntawm koj npauj txij hauv qab no yuav plam cov tis los yog muab nws tom ntej nrog ib tus pin. Qhov no yog ib lub caij zoo taw tes kom paub tias tej thaum kis tus kab mob los yog tsis raug thiab ib “cua tshuab” qhib mus xws li ib cov hauv qab no zoo tshaj. Yog hais tias koj muaj ib keev xeeb mus rau ib tug me quav thiab ilv npauj koj yuav tsum tau taub los txav cov tis nraim thiab cia tus zoo li qub ntawm npuas dej cia rau hauv qhov chaw tuav. Qhov no pub rau ib tug dlej saib ntawm lub hindwing tsis risking puas me ntsis uas yuav tau los thaum txoj mounting.
- Tus npauj ntxeev tiaj kus thiab txoj hauj lwm li tusyees thiab li flush li sai tau rau tus ua npuas ncauj. Koj yuav siv los xij loj ntawm npuas dej rau koj kis – cov ntshiab tas yog txoj zoo tshaj rau daim teb siv xwb (tshwj xeeb tshaj yog vim lawv pib tuaj dua lawm).
- Tus PIN los siv me me strips tracing ntawv tus tis, raws li rau ntawm lub Rooj Tswjhwm Saib cov kis loj tuaj.
- Siv tau ib tus pin minuten gripped nruj nreem ntawm koj ncais, zes tus tis rau tus ua tib yam. Costa rau ntawm tus forewing yuav muaj ib tug me me curled caj uas yooj yim heev tswj mus tis tsis tau muab tej qhov. Yog koj muaj hmoo tus tis yuav nyob coupled thiab kev tsis haum ntawm lub hindwing yuav tsum. Ib zaug nyob rau hauv qhov chaw tuav daim ntawv nrog koj cov ntiv tes thiab pin rau hauv qhov chaw.
- Tus npauj yuav tsuas muab ob peb hnub kom qhuav. Raws li cov txheej txheem no yuav siv sij hawm ntau xyaum thiab tsis tau txaus rau ib txhia. Thaum uas xyaum txaus koj yuav kis tau tus micro sai dua ib macro – thiab mus nrog nrws superior deb. Txawm li cas los, muaj ib txhia yuav xav tej cov pob me me grooves ua npuas dej thiab feeb ntau kis kom tsis txhob muaj muab uas muaj tshwm sim nrog ib tug upside-down lep. Ib tug koom mount plam pes tsawg yuav luag nplai – tsis muaj dab tsi ntau tshaj li yuav ua li cas txhua zaus – thiab nws txhob muab qhov tis.
Soj ntsuam!
Tsab xov xwm zoo kawg thiab – txawm ho kuv tsis kam nrog npaub, Kuv yeej xav pom lub hom kev kawm uas yog siv lwm tus.
Kuv yeej nyiam koj series ntawm cov khoom caag – Kuv zaum mam mus ua ib yam dab tsi zoo rau kab.
Piav yav tom ntej, Chris. Tsaug rau muab qhov no ua ke thiab ntaus tej ncauj lus nws.
Kuv sim nws thiab kuv txaus siab heev rau cov ntsiab. ua tsaug ntau!
Kuv xav tias kuv muaj txog no nyeem nag hmo, ua ntej kuv obliterated ib tug mus rau hauv me me quav, shredded smithereens, thiab tsis tau muaj hnub no. Tom qab cov obliterating twb twb noj chaw. Huag zoo, lwm zaus.
Oooh, qhov no yog li lawm. Tseem yuav tsum tau xyaum, tab sis tus obliteration yog cia mus yam tsawg mus khaws!
Zoo siab koj nyob nraum muab rau nws ib zaug! Kuv pog mus hnov txog deb sab qaum teb yog leps.
Ntawm no yog qhov…Kuv nco yog ib qho tseem ceeb heev tshaj plaws daim lep % xwb tej cuab yeej…ib lub teeb mag. Thaj, txawm muaj 24 teev hnub ci ua haujlwm wel, tab sis yog vim tsis muaj shipping ib down proper ib yig. Li ntawd, kuv txwv rau kuv aerial nqa, uas yog tiag limiting pes tsawg thiab hom ntes kuv”m tau. Yog koj muaj tswv yim dab tsi kuv yuav rig muab liab qab-cov pob txha muag khoom kho vajtse-yam khoom los ua ib cuab, Kuv yog cov pob ntseg.
Tab sis npog, loj heev, lub leps zoo li yuav tsis sab ob hom crisis: smallish butterflies (arctic-yam satyirds thiab cov pierids daj, tej nymphalids), thiab ntau ntau nti pom kev me me quav grey npaub uas kuv tsis tau sim muab ib lub npe ntawd tsis tau (vim npaub hem kuv…thiab kuv tsis muaj hauv lub lag luam muaj).
Zoo lub cuab lub teeb yog teeb saum tej thoob rau ib funnel tsi tsi muaj. Tsis yog lub teeb thiab hwj huam qhov yog qhov tshaj plaws yam, tiam sis txhua Radon tau UV teeb ntawm tej tsi. Yog muaj ntau lub ntuj nyob saud? Nws yuav tau ib qho teeb meem uas los ntawm cov ntxiab txhuav – tiam sis yog hais tias tsis tau ces nws yuav tsum tau yooj yim heev rau jerry-rig ib txhia tsawm tsawv thiab funnel hla ib lub thoob (Txawm hais tias nws yog ntawv!). Koj yeej tseem qoo ua ke ib ceev “skinner” mag style. Koj yuav tau txais ib ntxhib lub tswv yim no: http://www.pwbelg.clara.net/mercury/case/index.html
Tab sis txhob siv lub funnel no xwb 2 tej daim uas ua rau ib tug / zoo rau saum ib lub phij xab.
Yog hais tias koj tau txais los pos huab nag ces koj puas tau xav txog lub khob rau hauv qab nrog ib hose uas sticks tawm sab los si nws. Tiam sis zoo tsis meej tau hauj lwm ua.
Thiab hav yuav xav kom muaj li npauj ID's! Yog li kuv yuav taw koj mus canadian npauj pej (uas koj sawv daws paub?).
Hmm. Kuv yuav zom rau hauv no. Yog kuv cov ntsiab teeb meem 1. tau qhov twg los zog (Kuv tsis muaj dab tsi kuv tau coj hauv lub teb, tsis generator. Ib lub roj teeb, saib tsam?) 2. Mas nws yeej tsis muaj UV teeb ntawm no (yuav muaj ib txoj kev incandescent ua tau? los yog ib tug noob coloured tej yam?) 3. Los nag…los nag yuav luag txhua hnub, yam tsawg kawg ib lub ntsis. Tab sis kuv yeej xav tau tej yam ua ntau tshaj li kuv tau (Nws yog ib qho heev, heev tsawg).
Roj teeb uas kuv siv – cov 12v lawnmower style. Incandescent tsis zoo heev, tab sis nws yuav zoo dua tsis muaj dab tsi. Tsis txhob daj/kab ntxwv/liab – lub zog dawb zoo. Ib floodlight tej zaum qee yam?
Koj yuav xyaum ua tej yam uas cov tshaj cum nteg qe thiab khob rau nag…kuv tsim lawm yeej muaj funnel nrog cov ntxaij nws tshev tawm ncaj qha ntawm cov thoob qab. LOS SIS, txhob siv ib nag ntws, koj yuav tso tuab loj dua qhov tseem tshaj plaws.
Yuav tsum tau duab! hmoov zoo.
Kuv qhov txheej txheem ib leeg yuav cia kuv mus kawm tiav rau lub sij hawm zoo haum.
Kuv yog ib tug me nyuam kawm ntawv Masters nyob ua kev tshawb fawb txog ntau haiv neeg lus npauj Shenandoah National Park UVA. Kuv muaj tsib lub cuab lub teeb nyob rau tom hav zoov, ntaiv plaub zaug thaum ntuj cuaj hlis. Kuv mam li muaj roughly 30 k nrws paub thaum finishes ntaiv kawm uas kawg nkaus, thiab microleps tau qhov bane kuv hnub pinning. Feem ntau muaj nyob rau hauv tej kev txom nyem mob tom qab ntaiv, nrom kus tuag rau hauv lub thoob kom txog thaum kuv yuav mus kev mus sau nws, ces cia thaum kuv tau lawv kom khov, tab sis kuv yuav tau lees tias ib sab yuav kis tau zoo txaus rau daim ID siv yam tshuaj no, txhob siv cov qauv txheem.
Tsaug!
Zoo siab kuv yeej pab! Wow – koj yeej paub txhua tus leps uas tuaj hauv lub teeb? Koj tseem tabtom cuv npe hom rau txhua yam? (suab nrov zoo ib yam li ib tug yog ib qhov me me PhD!) Tua kuv tus e-mail yog hais tias koj muaj lus nug dab tsi ntxiv.
Thov txim, lub hli vim kuv muab lo kuv muaj tibneeg hu tauj coob xov kawm. Cov kev kawm tiav, Kuv refocused rau kuv tshawb fawb, li ntawd, kuv nrhiav tau kuv txoj kev nram qab no.
Peb yuav tsis muaj nrog ib tse neeg nraim kev microlep yog tias peb tsis tau ua kom yooj yim (i.e. tsis muaj qhov chaw mos dissection). Peb debated morphospecies, tiam sis kuv tsis xav tias zoo heev rau cov nyiaj ntawm cov ntaub ntawv uas plam, ntxiv rau kuv pab ib yam nkaus thiab arbitrary nrhiav. Kuv yuav tsum sau tag nrho los ntawm npaub North of Mexico ntawm tes, ces peb mam saib dab tsi tawm ntawm nws!
nyob zoo. Qhov txuas mus rau tus txheej txheem relaxing npaub ntawm nplooj ntawv no zoo li yuav tawg.
Ua tsaug! Qhov txuas tus yuav tsum raug muab daws tam sim no.
Kuv tuaj los Chris, tsaug heev npaum li cas rau tag nrho cov nqi lus uas nws kuj yog ib pab loj loj rau kuv. Puas ntsib ib co lus nug hais tom qab nyeem cov saum no. Qhov no yog implied saum toj no tab sis cia li mus xyuas:
1) Cas koj yuav tsis muab ob npaug rau-mount puas tsav puas yam > 20 hli? Hauv yuav koj cia li muab ib #1 tus PIN ncaj ntawm lub hauv sab qab plab nyiam ib qho tshuaj tua kab yog ib yam dab tsi lawm 20 hli?
2) Npaub uas yog pinned siv Terry tus txheej txheem muab zwm rau hauv lub thawv yas? Los yog hauv tej kis ua koj ob-mount lawv thiab muab cia rau hauv kab txheem drawers?
3) Nws suab zoo li ntawm cov ntawv saum toj no kom koj so tag nrho tus npauj nrws uas koj sau?— yog qhov tseeb? Kuv twb nyob hauv lub tswv yim tias koj tsuas tau los so nrws yog hais tias lawv muaj quav, tiam sis tej zaum tej nyiaj mus relaxing tag nrho xwb Txawm li cas los yog lawv tau tuag rau ib nqi tas sij hawm? Kuv siv los mus sau ib Rev ntawm cov neeg uas nyob rau hauv ib lub thawv tua los ntawm blacklighting thiab ces pinning lawv nyob rau hauv 24 xuaj moos los sis li (hnub tom ntej no feem ntau yog tom qab lub tsev rov qab). Xav koj tseem so kom txaus rau qhov no?
Kuv tuaj los Rick-
1) Yog nyob, Kuv muaj ib npaj cov tshuaj thicker minuten pins ntawm Watkins me me & Doncaster kuv tej zaum kuj yuav siv rau Pyraloids. Thiab kuv feem ntau yuav nrhiav kuv ob-mounting tus ilv bodied Geometridae Sterrhinae zoo ib yam li. Kuv tej zaum kuj hloov ntawm ob chav mounting los siv tau ib tus pin uas tag nrho tej yam li Ethmia. Txawm hais kuv muab ua kom puas siv me dua txhua yam tsis muaj #1 tus PIN.
2) Cov no feem ntau yog hauv cia ib ntus xwb, ces tus zoo tagnrho kawg lo lus uas peb tseem muaj ob mount thiab daim ntawv qhia txog kev ntaus cim. Txawm tias nws yuav kis tsis tau nws yog npaum li cas zoo li tsis muaj qhov no ua li cas! Kuv tseem npaj siab yuav mus kis tej koob yog tias kuv tau.
Kuv nyuam qhuav nrhiav tau cov lus teb rau lo lus nug peb hauv koj qhov lus teb rau cov lus koj relaxing nrws ntaw — xws li tsis tau teb ntawd ib! 🙂
Kheej tsaug Chris!
Kuv pom tias qhov uas lub cev ntawm tus butterflies yuav qhuav qhawv. Kuv yav dhau los mas tsuas ua haujlwm 3 hnub qub nrws uas txhom tau hauv kuv lub vaj. ( lawv tau yooj yim– tsis muaj rehydrating tom qab ib hmo hauv lub lub freezer.) Ntawm tus loj bodied ones kuv yuav, hopefully, lawv yuav rehydrate tsis tau moldy, tom qab 24 teev lawv cov pob txha qhuav rau qhov uas lub cev. Kuv tsis muaj koob txhaj tshuaj. Kuv yuav ua tus boiling dej thiab cov sponges. Kuv ntseeg tias thaum nriav hairstreak butterflies lawv yuav tsum twb kis. Kuv tsis tau lawv 3/4 kis .. tsis ncaj nraim thiab thaum mounted lawv zoo li lawv nyob rau hauv lub davhlau. Optimum tsis tab sis zoo haum. Zoo dua lawv rhuav kuv piv txwv.
ib lub inverter rau nqe lauj yuav ib lub tsheb roj teeb rau muab qhov xav kom tau AC rau teeb great xwb. yuav tsum tau txais ib tug uas yog nyob rau yav 50% ntau tshaj cov chaw nrhiav tswvyim los ntawm teeb. TSIS TXHOB SIV COV ROJ TEEB RAU HAUV KOJ LUB TSHEB AS YOG TIAS NWS TAU SIV MUS NTEV LAWM KOJ YUAV TSIS TAU KOJ LUB TSHEB PIB!!! Yog hais tias koj tsis mloog koj yuav khaws lub tsheb khiav, tiam sis yuav siv ntau roj/petrol. Kuv muaj ib cais roj teeb uas muaj isolator mounted kom lub tsheb yuav ntawd them tab sis kuv yuav siv nws mus txog rau thaum nws yuav tsis faiv fab tso lub teeb muaj; lub tsheb no yog pib muaj cai tuaj.
Kuv kuj muaj ib qhov me me 1Kw generator. Nws yog roughly 30 x 30 x 30 cm los yog 1 x 1 x 1 taw thiab muaj kev haum pheej yig ris rau hauv tej qhov chaw uas kuv jeep yuav tsis mus.